In contrast, in a long-term survival experiment, several injections administered over consecutive days can label a cohort of cells that are subsequently identified as a single group, not taking into account the age difference between the cells marked by the first and the last injection.This inherent limitation of Brd U labeling implies that each animal can only contain a population of cells marked, as homogeneous as possible in terms of age, precluding any distinction between cells of very different ages.
Therefore, Brd U administration must be sufficiently discrete in time (depending on the experimental design) so that cell populations to be marked by Brd U are also consistent in terms of age.After the animal is sacrificed, staining is detected by immunohistochemistry using antibodies specifically directed against the analog.Brd U has been the marker of choice in recent years for several reasons, in part because this method requires no radioactivity unlike the use of tritiated thymidine, which for decades was used to label dividing cell populations during brain development.Your access to the NCBI website at gov has been temporarily blocked due to a possible misuse/abuse situation involving your site.This is not an indication of a security issue such as a virus or attack.Therefore, the physiological and quantitative analysis of neuronal subpopulations at different ages is critical to studies of neurogenesis.
Such approaches allow cells of different ages to be identified by labeling them according to their probable date of birth.
Furthermore, Brd U staining is readily detected by immunohistochemistry.
The only significant drawback of this technique is the requirement to unmask the epitopes recognized by the primary antibody against Brd U using hydrochloric acid.
This issue is particularly important in short experiments (i.e., days).
If the animal is injected with Brd U over n days, and sacrificed 1 week after the last injection, the cohort of labeled cells is considered to be between 7 and 7−n days old.
It will be important to note here that these cell populations can be traced using relatively specific markers (Figure 1).